Coding
Part:BBa_K1897003:Design
Designed by: Choi Yan Ru Group: iGEM16_NUS_Singapore (2016-10-09)
HasS antisigma factor coding sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 34
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 317
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 72
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
NIL
Source
The HasS gene is originally found in the Serratia marcescens. We synthesised the part from the Serratia marcescens genome, obtaining the gene sequence from the National Center for Biotechnology Information (NCBI) database (http://www.ncbi.nlm.nih.gov/nuccore/X81195.2).
References
Biville, F., Cwerman, H., Létoffé, S., Rossi, M. S., Drouet, V., Ghigo, J. M., & Wandersman, C. (2004). Haemophore‐mediated signalling in Serratia marcescens: a new mode of regulation for an extra cytoplasmic function (ECF) sigma factor involved in haem acquisition. Molecular microbiology, 53(4), 1267-1277.